Ö÷²¥ÓÕ»ó

Object moved to here.

Ö÷²¥ÓÕ»ó :: Pilot & Feasibility Program Application

Ö÷²¥ÓÕ»ó


Molecular Characterization of Detrusor Interstitial Cells in Diabetic Detrusor Overactivity
Summary Data Summary
Applicant Lee, Haeyeong
E-Mail Address hylee@unr.edu
Project Title Molecular Characterization of Detrusor Interstitial Cells in Diabetic Detrusor
Overactivity
CBU ID 19AU3978
External SubContract ID 32307-51
Diabetic Complication Uropathy
Funding Program Group Pilot & Feasibility [PF2019]
Abstract Diabetic detrusor overactivity is the most common and costly complication of
Diabetes Mellitus (DM). Estimates of the prevalence of diabetic bladder
dysfunction range from 25% to 83%. DM causes two phases of change in bladder
function: In the early phase of DM, detrusor overactivity is observed, but later
stage of DM shows underactive bladder in animal models and human patients. Many
tissue-level studies have been performed to investigate the mechanisms of
diabetic bladder dysfunction, but these mechanisms remain unclear at present. We
have found a novel type of interstitial cell in detrusor muscles of the bladder
recently. These cells were first identified with antibodies against
platelet-derived growth factor receptor-a (PDGFRa), and we refer to them by this
chemical coding, PDGFRa+ cells. Detrusor PDGFRa+ cells are an important
mechanosensitive element that is activated during bladder filling. These cells
show high expression of TRPV4 channels and small conductance Ca2+-activated K+
(SK) channels in comparison to smooth muscle cells (SMCs) which have minimal
expression of both channels. During bladder filling activation of TRPV4 channels
increases Ca2+ influx into PDGFRa+ cells and this activates SK channels, leading
to stabilization of membrane potential in the SMC/PDGFRa+ cell syncytium. In
this project, we will focus on exploring molecular mechanisms of early diabetic
detrusor overactivity. We will target specific cellular mechanisms to explain
the early phases of diabetic detrusor overactivity. The overarching hypotheses
of this project are that changes in membrane excitability-regulating genes of
PDGFRa+ cells and loss of PDGFRa+ cells of diabetic detrusor muscles induce
detrusor overactivity due to disruption of membrane stabilization during
filling. The project is novel and timely because: i) PDGFRa+ cells have recently
been discovered and nothing is known about their fate in DM; ii) cellular
remodeling of detrusor muscles has never been characterized in DM. Molecular
characterization of the changes in PDGFRa+ cells in DM bladder will provide new
ideas about the etiology of diabetic bladder dysfunction and may identify new
rationales for therapies.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel
Salary Total Costs 60872
Supply Total Costs 8573
Equipment Total Costs 0
Travel/Other Total Costs 0
Direct Costs 69445
Indirect Costs Proposed 0
Total Costs Proposed 69445
Total Costs Approved 100000
Start Date 11/1/2019
End Date 4/30/2021
IFO Name Hart, Charlene
IFO E-Mail Address ospadmin@unr.edu
IACUC/IRB No. 99999
IACUC/IRB Institution University of Nevada-Reno
Entity ID No.
Report Request Date 4/30/2021
T1D NO
TypeCount
Invoices 13
Progress Reports 2
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  19AU397832307-51University of Nevada-Reno9/18/2020$9,305.71$4,094.50$13,400.21$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno8/4/2021$14,228.29$6,260.44$20,488.73$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno8/18/2020$2,313.82$1,018.07$3,331.89$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno6/11/2020$1,201.89$528.83$1,730.72$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno5/7/2020$778.99$342.76$1,121.75$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno4/30/2021$3,923.70$1,726.42$5,650.12$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno4/10/2020$250.00$110.00$360.00$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno3/18/2020$375.00$165.00$540.00$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno3/17/2021$3,833.70$1,686.82$5,520.52$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno2/18/2021$4,293.70$1,889.22$6,182.92$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno12/16/2020$6,975.92$3,069.40$10,045.32$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno10/19/2020$8,495.16$3,737.89$12,233.05$0.06View PDF
  View  19AU397832307-51University of Nevada-Reno1/21/2021$13,468.56$5,926.15$19,394.71$0.06View PDF
Click here to cancel and return to funding program application

*Author:
*SubContract:
*Select File:

Click browse and select the file to upload.
(Please upload ONLY TXT, Image Files (not histolgy), PDF, XLS, XLSX, DOC, or DOCX files.)