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Complex, 3D Skin-like Tissues for Preclinical Testing of Diabetic Ulcer Therapy
Summary Data Summary
Applicant Garlick, Jonathan
E-Mail Address jonathan.garlick@tufts.edu
Project Title Complex, 3D Skin-like Tissues for Preclinical Testing of Diabetic Ulcer Therapy
CBU ID 18AU3889
External SubContract ID 32307-26
Diabetic Complication Wound Healing
Funding Program Group Pilot & Feasibility [PF2018]
Abstract We seek to develop more complex and predictive, in vitro, human 3D tissue wound
healing models for preclinical drug screening that will effectively test the
safety and efficacy of novel therapeutics for Diabetic foot ulcers (DFU). This
will help meet the urgent need to develop next generation treatments for these
and other complications of diabetes by providing more cost-effective ways of
predicting the success or failure of drugs designed to treat DFUs before they
enter human clinical trials. To fill this critical need, our immediate goal is
to characterize and test diabetes-specific, preclinical wound healing models
fabricated as in vitro, 3D tissues. We will develop a novel DFU wound repair
platform that will have 3 important, new features: 1- harbor DFU fibroblasts and
macrophages from a spectrum of Type II DFU patients, 2- a higher throughput
format (24 well) than existing tissues and 3- will eliminate all animal products
and incorporate only human cells and tissues. This will improve upon our
existing DFU wound healing models by creating more complex tissues that will be
an important advance over currently available monolayer, tissue cultures. We
expect to learn that novel DFU patient-specific, 3D tissue wound models that
mimic chronic wounds like DFU can be optimized as a critical step towards
developing high-impact, high-throughput human preclinical drug testing platforms
for DFU through two SPECIFIC AIMS: 1- To optimize human, DFU patient-specific 3D
tissue models harboring primary DFU-derived fibroblasts and macrophages and 2-
To test, characterize and optimize these 3D tissue models of DFU wound
re-epithelialization. Our research is based on our previous work showing that:
1- DFU patient-derived fibroblasts generate 3D tissue models that simulate
defective wound healing of DFUs in vivo, 2- primary, DFU-derived macrophages
retain their M1/M2 polarization states when incorporated into 3D tissue models
and 3- 3D skin-like tissues can be fabricated with fibroblasts, macrophages and
extracellular matrix in a 24-well, tissue format. The success of our strategy
will significantly improve our ability to identify biomarkers of DFU and
efficiently screen candidate drug for DFU treatment at an early stage in their
development in a reproducible and convenient tissue model. By successfully
developing small-format, 3D tissues, we will lay the groundwork to incorporate
these tissues into “tissues on chips†formats. This will shift preclinical drug
screening for DFU towards the dissemination, scale-up and commercialization of
preclinical wound healing models that will prioritize and streamline drug
discovery for DFUs so they can safely be used in human clinical trials.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel Jonathan Garlick
Salary Total Costs 42111
Supply Total Costs 18378
Equipment Total Costs 0
Travel/Other Total Costs 0
Direct Costs 60489
Indirect Costs Proposed 39318
Total Costs Proposed 99807
Total Costs Approved 99807
Start Date 11/1/2018
End Date 10/31/2019
IFO Name Donaghey, Colleen
IFO E-Mail Address colleen.donaghey@tufts.edu
IACUC/IRB No. 99999
IACUC/IRB Institution Tufts Medical Center
Entity ID No. 00004517
Report Request Date 11/29/2019
T1D NO
TypeCount
Invoices 9
Progress Reports 1
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  18AU388932307-26Tufts Medical Center9/9/2019$6,788.05$4,412.23$11,200.28$0.17View PDF
  View  18AU388932307-26Tufts Medical Center8/9/2019$6,018.29$3,911.89$9,930.18$0.17View PDF
  View  18AU388932307-26Tufts Medical Center7/27/2019$5,074.92$3,298.70$8,373.62$0.17View PDF
  View  18AU388932307-26Tufts Medical Center6/9/2019$2,857.14$1,857.14$4,714.28$0.17View PDF
  View  18AU388932307-26Tufts Medical Center5/9/2019$3,830.55$2,489.86$6,320.41$0.17View PDF
  View  18AU388932307-26Tufts Medical Center4/16/2019$8,458.20$5,497.83$13,956.03$0.17View PDF
  View  18AU388932307-26Tufts Medical Center12/16/2019$7,514.53$4,884.44$12,398.97$0.17View PDF
  View  18AU388932307-26Tufts Medical Center11/6/2019$12,812.94$8,328.41$21,141.35$0.17View PDF
  View  18AU388932307-26Tufts Medical Center10/7/2019$7,134.37$4,637.34$11,771.71$0.17View PDF
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