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Transformative Tools for Imaging Tissue Ultrastructure in Diabetic Kidney Disease
Summary Data Summary
Applicant Vaughan, Joshua
E-Mail Address jcv2@uw.edu
Project Title Transformative Tools for Imaging Tissue Ultrastructure in Diabetic Kidney
Disease
CBU ID 17AU3794
External SubContract ID 32307-6
Diabetic Complication Nephropathy
Funding Program Group Pilot & Feasibility [PF2017]
Abstract This proposal seeks to apply and adapt the next-generation super-resolution
optical microscopy method expansion microscopy (ExM) to the study of human
diabetic kidney disease, the most common cause of end-stage renal disease in the
U.S. In ExM, fluorescent labels on fixed specimens are linked to a swellable
polymer hydrogel that is physically expanded with remarkably low distortion. The
process allows features closer than the diffraction limit of light (~250 nm) to
become resolvable in the expanded specimen and achieves ~65 nm spatial
resolution on thick or thin specimens with ordinary confocal microscopes. This
greatly improved spatial resolution is sufficient to reveal key ultrastructural
features of the kidney including glomerular foot process width and glomerular
basement membrane width while also providing molecular details such as
extracellular membrane composition or presence of immune deposits, etc.
Importantly, ExM makes use of standard optical microscopes that are already
familiar and widely available, together with inexpensive sample processing
methods, so that the method has the potential to become widely used in research
and clinical settings for the study or diagnosis of kidney diseases, including
DKD. Our specific aims are: 1) to develop and optimize procedures to study fresh
kidney specimens using ExM; 2) to adapt these procedures to study archived
paraffin-embedded tissue by ExM; and 3) to perform correlative ExM and electron
microscopy imaging as a robust validation of our methodology.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel Najafian, Behzad; Alpers, Charles
Salary Total Costs 29556
Supply Total Costs 16000
Equipment Total Costs 0
Travel/Other Total Costs 20934
Direct Costs 66490
Indirect Costs Proposed 33508
Total Costs Proposed 99998
Total Costs Approved 100000
Start Date 11/1/2017
End Date 10/31/2019
IFO Name DeFries, Kirsten
IFO E-Mail Address gcahelp@uw.edu
IACUC/IRB No. 9999
IACUC/IRB Institution University of Washington
Entity ID No. 91-6001537
Report Request Date 11/30/2019
T1D NO
TypeCount
Invoices 15
Progress Reports 1
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  17AU379432307-6University of Washington9/11/2018$4,651.38$2,558.27$7,209.65$1.00View PDF
  View  17AU379432307-6University of Washington8/9/2018$10,537.58$5,795.67$16,333.25$1.00View PDF
  View  17AU379432307-6University of Washington7/12/2018$5,170.02$2,047.33$7,217.35$1.00View PDF
  View  17AU379432307-6University of Washington6/11/2018$2,932.72$1,612.99$4,545.71$1.00View PDF
  View  17AU379432307-6University of Washington5/9/2019$4,975.21$2,736.36$7,711.57$1.00View PDF
  View  17AU379432307-6University of Washington5/9/2018$2,814.18$1,547.81$4,361.99$1.00View PDF
  View  17AU379432307-6University of Washington4/10/2019$9,667.96$1,755.15$11,423.11$1.00View PDF
  View  17AU379432307-6University of Washington4/10/2018$1,688.95$928.92$2,617.87$1.00View PDF
  View  17AU379432307-6University of Washington3/20/2018$6,720.98$2,344.38$9,065.36$1.00View PDF
  View  17AU379432307-6University of Washington3/11/2019$2,725.94$1,493.42$4,219.36$1.00View PDF
  View  17AU379432307-6University of Washington2/13/2019$4,280.86$2,354.47$6,635.33$1.00View PDF
  View  17AU379432307-6University of Washington12/24/2019---$1.00View PDF
  View  17AU379432307-6University of Washington12/20/2018$4,149.45$2,282.20$6,431.65$1.00View PDF
  View  17AU379432307-6University of Washington10/10/2018$3,541.86$1,948.04$5,489.90$1.00View PDF
  View  17AU379432307-6University of Washington1/9/2019$4,346.38$2,390.52$6,736.90$1.00View PDF


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