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A new in vivo model for imaging axon dynamics in injured diabetic zebrafish
Summary Data Summary
Applicant Rieger, Sandra
E-Mail Address srieger@miami.edu
Project Title A new in vivo model for imaging axon dynamics in injured diabetic zebrafish
CBU ID 12GHSU179
External SubContract ID 25732-3
Diabetic Complication Neuropathy & Neurocognition
Funding Program Group Pilot & Feasibility [PF2012]
Abstract Diabetes mellitus currently affects over 26 million adults in the United States
and 285 million people worldwide. These numbers are expected to nearly double in
the next two decades. Neuropathy-mediated wound healing complications are a
major problem in diabetes, with diabetic foot ulcerations (DFU) preceding
approximately 84% of lower limb amputations. Long-term intensive care and
consequences of amputation place immense burdens on individuals and their
families as well as healthcare systems. While interdisciplinary clinical groups
have made certain advances in DFU prevention and control of the disease, higher
success could be achieved through a better understanding of the underlying
causes. This lack of knowledge mostly derives from the complex etiology of DFU.
Although the use of mammalian model organisms has proven to be highly important
for the investigation of diabetes-associated complications, they are subject to
technical limitations and high costs. New low-cost model systems are required
allowing for in vivo analyses of the complexity of axon-wound interactions in
live animals. With the power of in vivo imaging new concepts underlying the
etiology of DFU could emerge. Here we propose to develop zebrafish larvae as a
new in vivo model for elucidating basic mechanisms of DFU. Zebrafish larvae have
several advantages over other model organisms in that they are optically clear
and develop rapidly. This makes them suitable for genetic and pharmacological
manipulations and for in vivo imaging. In addition, glucose metabolism in larval
and adult zebrafish is highly similar to humans. Thus zebrafish larvae are ideal
for investigations of diabetic complications. The goal of this proposal is to
combine our previously established axon regeneration model in zebrafish larvae
with genetic ablation of insulin-secreting pancreatic b-cells to analyze the
behavior of acutely injured and neuropathic cutaneous axons under hyperglycemic
conditions. This model will allow us to address basic questions that are key to
understanding DFU etiology: 1) is there a temporal relationship between b-cell
death and onset of neuropathy? 2) Do acutely injured cutaneous axons regenerate
under hyperglycemic conditions? And 3) does acute injury initiate or aggravate
neuropathy? Understanding these basic mechanisms will be fundamental for
investigations on neuropathy-related chronic wound formation. This work has the
potential for future pharmacological studies in zebrafish larvae with the goal
to identify drugs that suppress the onset of neuropathy and prolong axon health.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel
Salary Total Costs 28160
Supply Total Costs 9000
Equipment Total Costs 0
Travel/Other Total Costs 8000
Direct Costs 45160
Indirect Costs Proposed 33870
Total Costs Proposed 79030
Total Costs Approved 79030
Start Date 10/1/2012
End Date 9/30/2014
IFO Name Lurvey, Claudine
IFO E-Mail Address clurvey@mdibl.org
IACUC/IRB No. 11-17
IACUC/IRB Institution Mount Desert Island Biological Island (MDIBL)
Entity ID No. 01-0202467
Report Request Date 1/1/3000
T1D NO
TypeCount
Invoices 22
Progress Reports 1
Protocols 4
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)9/26/2014$100.34-$100.34$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)9/25/2013$3,588.31$2,619.47$6,207.78$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)8/16/2013$2,168.87$1,583.28$3,752.15$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)7/31/2014$119.37-$119.37$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)7/17/2013$1,912.78$1,396.33$3,309.11$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)6/30/2014$765.30-$765.30$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)6/19/2013$2,017.96$1,473.11$3,491.07$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)5/21/2013$2,538.80$1,853.32$4,392.12$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)5/20/2014$3,662.12-$3,662.12$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)4/22/2014$4,377.97-$4,377.97$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)4/16/2013$3,057.93$2,232.29$5,290.22$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)3/31/2014$3,031.66$2,213.11$5,244.77$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)3/22/2017---$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)3/18/2013$3,204.52$2,339.30$5,543.82$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)2/22/2013$2,258.55$1,648.74$3,907.29$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)2/19/2014$2,241.93$1,636.61$3,878.54$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)12/30/2013$3,005.34$2,193.90$5,199.24$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)11/29/2013$1,050.59$766.93$1,817.52$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)10/24/2014$72.66-$72.66$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)10/23/2013$1,354.85$989.04$2,343.89$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)1/22/2013$5,078.79$3,707.52$8,786.31$1,093.83View PDF
  View  12GHSU17925732-3Mount Desert Island Biological Island (MDIBL)1/17/2014$3,897.82$2,845.41$6,743.23$1,093.83View PDF


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