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Publication

Laser capture microdissection of kidney tissue.

Authors Woroniecki RP, Bottinger EP Submitted By Erwin Bottinger on 5/2/2011 Status Published Journal Methods in molecular biology (Clifton, N.J.) Year 2009 Date Published 3/20/2009 Volume : Pages 466 : 73 - 82 PubMed Reference Abstract Kidney tissue laser capture microdissection (LCM) is of great clinical relevance since genome wide studies on total kidney messenger RNA (mRNA) potentially miss important factors involved in the pathogenesis of the disease in glomeruli and tubules. This technique is readily applicable to study mRNA from isolated glomeruli and tubules of human kidney biopsy material. In this chapter we present a "cook-book" practical approach of utilizing LCM in combination with RNA isolation technique in downstream applications in nephrology.

Investigators with authorship
Name Institution Erwin Bottinger Mount Sinai School of Medicine

Complications
All ComplicationsBioinformatics BoneCardiomyopathy CardiovascularGastro-Intestinal (GI) NephropathyNeuropathy & Neurocognition Pediatric Endocrinology Retinopathy UropathyWound Healing