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Hyperglycemia and white blood cell biology
Summary Data Summary
Investigator Goldberg, Ira
Description Several complications of diabetes may be exacerbated by inflammation. Whether
this is due to hyperglycemia, defective insulin actions, or other metabolic
alterations of diabetes is unknown. In addition, both local tissue inflammation
and changes in the biology of circulating white blood cells (WBCs) could lead to
pathological changes in organs and vascular tissues. In this regard, we have
recently found that loss of WBCs from atherosclerotic mouse lesions is defective
in the presence of streptozotocin diabetes. Moreover, we provide Preliminary
Results showing that changes in circulating WBCs that occur with diabetes are
corrected by glucose reduction using inhibitors to the sodium glucose
co-transporter 2 (SGLT2). Thus, the studies proposed will allow us to
“understand the possible in vivo efficacy of a promising new therapy for
diabetes”, glucose reduction via inhibition of SGLT2. This Pilot application has
two aims: Aim 1. To determine if hyperglycemia reduction leads to an alteration
in the inflammatory state of circulating monocytes and neutrophils. Aim 2. To
determine whether glucose reduction in STZ-treated LDL receptor knockout mice
with atherosclerosis will reduce the inflammatory profile of lesional CD68+
cells. Techniques to lower glucose in diabetic mice, isolate circulating
monocytes subsets (Lys6-C hi and lo) and granulocytes by FACS, and vascular wall
CD68+ cells by laser capture microdissection are in hand. We expect that our
studies will clarify the importance of hyperglycemia alone in altering the
inflammatory status of circulating and vascular WBCs.
Status Completed
Public Release 10/1/2014
Data Collected? Data will not be collected for this catalog item
Species M. musculus
Animal Age Measured In: week(s) post-natal (w)
Data Analysis
TypeCountReleased
Animals0-
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Phenotype Assays0-
Phenotype Measurements00
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Histology Images00
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